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US Patent 12006519 Polymerase mutants and use with 3′-OH unblocked reversible terminators

Patent 12006519 was granted and assigned to Agilent Technologies on June, 2024 by the United States Patent and Trademark Office.

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Patent abstract

Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

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