16s Sequencing

16s sequencing is the sequencing of the 16s rRNA gene for taxonomic resolution in microbiome analysis. Due to variability in 16S, 18S and ITS (internal transcribed spacer), these regions are commonly used for amplicon sequencing for microbial identification and phylogeny studies of samples from complicated microbiomes or environments. The 16S rRNA found in prokaryotes is homologous to 18S rRNA found in eukaryotes. The 16S rRNA is the rRNA in the small subunit of the ribosome.