The sequencing of the 16s rRNA gene for taxonomic resolution in microbiome analysis
CD Genomics is offering cheaper, faster and advanced 16S/18S rRNA and ITS amplicon sequencing technology with high quality data and satisfying results by using Illumina high-throughput sequencing instruments, enabling you to explore the diversity of microbial communities.
16S/18S/ITS Amplicon Sequencing has now been a well-established method for microbial identification and phylogeny studies of samples from complicated microbiomes or environments. In addition to next-generation sequencing platforms, CD Genomics also provides full-length 16S/18S/ITS amplicon sequencing by using PacBio SMRT sequencing technology.
As depicted in Figure 1, the beta diversity analysis discriminated HNSCC from control samples. PCA (Principal Component Analysis) reveals that the microbial communities in HNSCC patients are significantly different from those in control samples. NMDS revealed that the microbial communities in HPV- oropharyngeal samples are significantly different from those in HPV- oral cavity patients.
The OTU network depicted in Figure 3 significantly discriminates the HNSCC samples from normal samples, and HPV negative from HPV positive samples. The total abundance of Streptococcus, Dialister, and Veillonella had a different dominance in tumor samples and control samples.
The sequencing of the 16s rRNA gene for taxonomic resolution in microbiome analysis