Bacterial Genome Sequencing

CD Genomics is providing PacBio Single Molecular Real-Time (SMRT) sequencing to increase your research method for bacterial genome sequencing. A comprehensive view of the bacterial genome, including genes, regulatory regions, IS elements, phage integration sites, and base modifications is vital to understanding key traits such as antibiotic resistance, virulence, and metabolism.

At CD Genomics, we are using the long-read PacBio Sequel platform to support researchers all over the word with bacterial de novo whole genome sequencing needs. Our bioinformatics analysis include: Genome assembly and polishing, gene prediction, genome annotation, and comparative species genomes analysis.

Currently, the complete sequence map of more than 90% bacterial strains can be constructed by making use of a combination of Illumina HiSeq and PacBio SMRT systems. Pacbio RS II system can achieve complete genome assembly even in the regions of high or low GC content, as well as repetitive sequences. The complete sequence map of the rest 10% bacterial strains can be achieved with Sanger sequencing data. CD Genomics has completed hundreds of bacterial genome assembly cases without gap.

A closed, high-quality genome sequence for C. autoethanogenum DSM10061 was generated using only using PacBio sequencing to achieve "0 Gap" assembly and without the need for manual finishing. But there are still many gaps in the genome obtained using Illumina and 454 sequencing platforms. C.autoethanogenum and C. ljungdahliiare were indistinguishable at the 16S rRNA gene level and had high scores for similarity. Through whole-genome sequencing, it was found that there were significant differences in CRISPR system, hydrogenase and other aspects between the two, which were difficult to detect through second-generation sequencing.