A microtome chuck including a mounting portion having a mating surface operable to removably attach the mounting portion to a sample sectioning device, and an electrical contact operable to electrically connect the mounting portion to a power source. The chuck further including a sample receiving portion coupled to the mounting portion, the sample receiving portion having a sample receiving surface dimensioned to receive a sample, a light source coupled to the sample receiving portion, the light source operable to emit a light from the sample receiving surface and through a sample positioned along the sample receiving surface, and circuitry electrically connecting the light source to the electrical contact of the mounting portion.