Patent attributes
An arrangement for light sheet microscopy including illumination optics with an illumination objective for illuminating a sample, located in a medium on a sample carrier aligned with respect to a plane reference surface, with a light sheet. The arrangement further includes detection optics with a detection objective. The arrangement also includes a separating layer system with at least one layer separating the medium from the illumination and detection objectives. The separating layer system is aligned with a base surface parallel to the reference surface and contacts the medium by this base surface. At least one detection corrective lens, for reducing those aberrations occurring as a result of light to be detected passing obliquely through interfaces of the separating layer system, is configured as free-form lens and is arranged between the detection objective and the separating layer system. Alternatively, it also forms the front lens of the detection objective.
