US Patent 10078078 Bead incubation and washing on a droplet actuator
Patent 10078078 was granted and assigned to Advanced Liquid Logic on September, 2018 by the United States Patent and Trademark Office.
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The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
US Patent 10078078 Bead incubation and washing on a droplet actuator
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