The present invention provides a transparent dual-sided display device and a driving method thereof. The transparent dual-sided display device includes a display panel including a plurality of sub-pixel groups that each include a front-side OLED sub-pixel, a back-side OLED sub-pixel, and an electrowetting sub-pixel and a plurality of multiplexer modules that each include three thin-film transistors and a timing controller is involved to control a multiplexer module and a source driver, wherein the source driver writes the front-side and back-side output data signals to the front-side OLED sub-pixel and the back-side OLED sub-pixel respectively and applies a reference voltage to the electrowetting sub-pixel, such that controlling can be, through the timing controller, a voltage controller to output a voltage value of the reference voltage to the source driver to control transparency or light-blocking of the electrowetting sub-pixel for simultaneously fulfilling transparent displaying and dual-sided displaying.