A birthing chamber assembly includes a housing. The housing is positioned at an intersection between a pair of vertical support surfaces. Thus, a feline may reside in the housing when the feline is birthing kittens. A lid is removably positioned on the housing to shelter the feline. The lid has an aperture extending therethrough to facilitate the feline to enter the housing. The aperture is spaced from the housing when the lid is positioned on the housing. Thus, the aperture inhibits the kittens from exiting the housing.