A bicycle transport case includes a container adapted to be opened and closed. The container includes a first outer side and a second outer side and has a chamber for receiving a bicycle frame defined by interior sides of the first and second outer sides. A retaining device configured to retain the bicycle frame at various angular orientations is pivotally and detachably connected to the container. The retaining device includes a main guide rail pivotally connected to the container about an axis and at least one retaining stand for retaining the bicycle frame movably mounted on the main guide rail and adapted to be locked in various positions on the main guide rail.