A detachable tray for a ladder includes a container for retaining supplies, a bracket assembly pivotally coupled to the container that is adapted to be releasably secured to the ladder, and a handle pivotally connected to the container to facilitate tray transport. The bracket assembly includes a rung engaging bracket that is movably connected to a base by a pair of transverse threaded members. A gap is defined between the bracket and the base that is dimensioned to fittingly receive one side rail of the ladder, the width of the gap being adjustable to accommodate side rails of varying widths. A support block is slidably coupled to a side of the base by a pair of posts. Together, the base and the support block apply a firm abutment force against the opposite side of the side rail than the bracket, thereby stabilizing the tray in place on the ladder.