The present invention provides a knapsack with a cooling device. The knapsack comprises a knapsack body and a cooling device. The cooling device includes an electric motor, a radiating assembly and a circulating pipe. The electric motor is electronically connected with the radiating assembly. The radiating assembly is communicated with the circulating pipe. The circulating pipe consists of multiple curved U type units that connects with each other. The back of the knapsack body is provided with an interlayer. The multiple curved U type units of the circulating pipe are tiled in the interlayer. The cooling device is disposed in the knapsack to reduce the temperature of the back of the knapsack and keep it cool. It resolves the problem that the wearer's back feels hot and sweaty when the wearer uses the knapsack in hot day.