A power-up control circuit is provided and includes a pull-down resistor, a pull-down switch, and an enable device. A first terminal of the pull-down resistor is electrically connected to a configuration channel pin of a USB Type-C connection port. The pull-down switch is electrically connected to a second terminal of the pull-down resistor and a ground node. The enable device is electrically connected to the pull-down switch and the configuration channel pin. When receiving a voltage detection signal which is transmitted by a dual-role port device through the configuration channel pin, the enable device turns on the pull-down switch to electrically connect the pull-down resistor to the ground node, so that the dual-role port device detects the pull-down resistor through the configuration channel pin and supplies power to the microcontroller through a power supply pin to keep the microcontroller in the power-up status.