A slide rail assembly includes a first rail, a second rail and a third rail. The third rail is mounted between the first rail and the second rail. The first rail includes a blocking structure. When the third rail is located at a retracted position with respect to the first rail, the blocking structure is used for preventing the third rail from being moved from the retracted position in a retracted direction. When the third rail is located at the retracted position, the second rail can be moved to a predetermined position in the retracted direction with a rear portion of the second rail being beyond a rear portion of the first rail by a distance.