A transmission interface with noise reduction function includes a first circuit and a second circuit, for transmitting a signal from the first circuit to the second circuit or from the second circuit to the first circuit. The first circuit includes a first sub-winding and a first wire unit, and the second circuit includes a second sub-winding and a second wire unit. When an electromagnetic noise passes through the first sub-winding and the first wire unit, two loop currents are respectively generated, and the currents have opposite directions to cancel each other so as to reduce the electromagnetic noise. Or, when an emitting current corresponding to the signal flows through the first sub-winding and the first wire unit, two magnetic fields are respectively generated, and the magnetic fields have opposite directions to cancel each other so as to reduce the electromagnetic interference.