US Patent 10920267 Isothermal amplification of oligonucleotides

Processes are disclosed that create derivative DNA molecules in large numbers under isothermal conditions, where amplification of a looped structure derived from a target is achieved by a process that separately or in combination includes (a) a displaceable probe that (b) may be captured in a separate site, optionally containing (c) components of an artificially expanded genetic information system, where the primers optionally contain (d) components of a self-avoiding molecular recognition system, and optionally (e) involving direct transfer of DNA or RNA from a sample from a capture medium with immobilized quaternary ammonium salts.