Patent attributes
A method performed in a liquid chromatography system that includes a metering device pushing a sample into a trap column. The metering device sucks in the sample from a sample reservoir, wherein the sucking in the sample from a sample reservoir precedes the step of pushing the sample into the trap column. The liquid chromatography system also includes a trap column and a metering device (100), wherein the system (1000) is adapted to assume a configuration allowing the metering device (100) to push a sample into the trap column (6) and wherein the metering device (100) is adapted to push the sample into the trap column (6) in this configuration, wherein the system (1000) is adapted to assume a configuration allowing the sample to be sucked into the system (1000) by means of the metering device (100). Furthermore, the invention relates to a use of the liquid chromatography system (1000) for liquid chromatography, in particular of high pressure liquid chromatography.