A fence post marking assembly for marking fence post locations includes a pair of pipes that is each positionable in an upright orientation on ground. In this way each of the pipes can mark the proposed location of a fence post. A pair of supports is each coupled to a respective one of the pipes. A rod is positionable in each of the supports thereby facilitating the pipes to be spaced a pre-determined distance apart from each other. In this way the rod can precisely space the proposed locations for fence posts apart from each other. A can of spray paint is provided to mark the proposed location of the fence posts.