A locking cooler assembly includes a cooler that can store perishable items. The cooler is comprised of a thermally insulating material to retain the perishable items at a preferred temperature. A divider is hingedly coupled to the cooler and the divider opens and closes the cooler. A lid is hingedly coupled to the divider and the lid and the divider define a storage space when the lid is closed for storing non-perishable items. A locking unit is coupled to the divider and the locking unit engages the lid when the locking unit is engaged to secure the objects stored in the storage space defined by the divider and the lid. A remote control is in remote communication with the locking unit to facilitate the user to remotely disengage the locking unit.