Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Andrew Emili0
Date of Patent
March 8, 2022
Patent Application Number
15768602
Date Filed
October 17, 2016
Patent Citations
Patent Citations Received
Patent Primary Examiner
Patent abstract
Described are optical methods and reagents for sequencing polypeptides. A probe that exhibits different spectral properties when conjugated to different N-terminal amino acids is conjugated to the N-terminal amino acid of a polypeptide. Sequentially detecting one or more spectral properties of the probe conjugated to the N-terminal amino acid and cleaving the N-terminal amino acid produces sequence information of the polypeptide. The use of super-resolution microscopy allows for the massively parallel sequencing of individual polypeptide molecules in situ such as within a cell. Also described are probes comprising hydroxymethyl rhodamine green, an isothiocyanate group and a protecting group.
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