A method of deploying plugging devices in a well can include a spacing between the plugging devices by controlling a ratio flow rates through intersecting pipes. A deployment apparatus can include an actuator and a release structure that releases the plugging devices into a conduit connected to a tubular string in the well. Another method of deploying plugging devices in a well can include operating an actuator, thereby displacing a release structure, and the release structure releasing the plugging devices into the well in response to operating the actuator. Another deployment apparatus can include intersecting pipes and a valve that selectively permits and prevents displacement of the plugging devices through one of the pipes.