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US Patent 11384335 Genetically-modified immune cells comprising a microRNA-adapted shRNA (shRNAmiR)

Patent 11384335 was granted and assigned to Precision BioSciences on July, 2022 by the United States Patent and Trademark Office.

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The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

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