Patent 11486004 was granted and assigned to Singular genomics Systems, Inc. on November, 2022 by the United States Patent and Trademark Office.
Disclosed herein, inter alia, are complexes, kits, and efficient methods of sequencing two strands of a double stranded polynucleotide. In embodiments, methods of sequencing a circular template polynucleotide are provided including generating immobilized extension products.