US Patent 11535674 Bivalent bispecific antibody hybrid protein expression and preparation methods
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Baohong Zhang0
Hua Jiang0
Huili Lu0
Jianwei Zhu0
Junsheng Chen0
Kai Ding0
Lei Han0
Lei Zhang0
Yueqing Xie0
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US Patent 11535674 Bivalent bispecific antibody hybrid protein expression and preparation methods
Article
The present disclosure discloses a method for expressing and preparing a bivalent bispecific antibody. In the present disclosure, each portion of a bivalent bispecific antibody and an immune hybrid protein thereof is respectively expressed in a suitable prokaryotic or eukaryotic cell system, separated and purified by high-performance affinity chromatography, and then spliced in vitro by trans-splicing reaction mediated by an intein, to prepare the bivalent specific antibody and an immune hybrid protein thereof.
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Patent Jurisdiction
Patent Number
115356740
Date of Patent
December 27, 2022
0Patent Application Number
160790030
Date Filed
December 16, 2016
0Patent Citations
Patent Primary Examiner
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