The invention discloses a locking structure of a cup. The locking structure comprises a cup body and a cup cover. The cup body is provided with a first buckle, and the cup cover is provided with a second buckle adaptive to the first buckle. The locking structure further comprises a lock catch which is movably arranged on the cup cover up and down. The lock catch is provided with a bolt. Correspondingly, the cup body is provided with a bolt hole adaptive to the bolt. The cup cover rotates on the cup body, so that the first buckle and the second buckle are matched. When aligned, the bolt falls into the bolt hole, thereby realizing rotary locking.