Disclosed in the present disclosure is a hard disk fixing device, including: a seating, which includes a base fixed on a main board and a first pair of elastic buckles perpendicular to the base and arranged at two sides thereof; a first supporting seat, which includes a main body and an extending portion extending from the main body, wherein the extending portion may be snap-fitted between the first pair of elastic buckles, a first guiding slot is provided inside the main body and a through hole is provided in a side surface of the main body, and the first guiding slot is in communication with the through hole; a first adjusting fastener, which includes a sliding rod and a limiting rod connected to the sliding rod.