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US Patent 11786872 Vitro evolution in microfluidic systems

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Contents

Is a
Patent
Patent

Patent attributes

Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
Patent Number
11786872
Patent Inventor Names
David A. Weitz
Andrew David Griffiths
Jerome Bibette
Darren Roy Link
Keunho Ahn
Date of Patent
October 17, 2023
Patent Application Number
16864619
Date Filed
May 1, 2020
Patent Citations
‌
US Patent 7390463 Microcolumn-based, high-throughput microfluidic device
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US Patent 8067159 Methods of detecting amplified product
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US Patent 8153402 Emulsion compositions
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US Patent 8252539 Microfabricated crossflow devices and methods
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US Patent 8257925 Method for detecting the presence of a single target nucleic acid in a sample
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US Patent 8278071 Method for detecting the presence of a single target nucleic acid in a sample
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US Patent 8278711 Semiconductor device and method of making the same
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US Patent 8318434 Method for introducing a sample specific DNA tag into a plurality of DNA fragments from a plurality of samples
...
Patent Primary Examiner
‌
Narayan K Bhat
CPC Code
‌
B01L 3/502746
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B01L 3/502753
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B01L 3/502776
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B01L 3/502784
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B01L 2200/0636
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B01L 2200/0673
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B01L 2300/0654
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B01L 2300/0816
...
Patent abstract

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

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