US Patent 11788155 Lung microbiome isolation and cultivation

Disclosed herein is a method to isolate and cultivate the lung microbiome obtained from bronchoalveolar lavage. The disclosed method involves lung microbiome culturomics based on three different media for both aerobic and fastidious anaerobic bacteria. The disclosed method allows for amplification of the lung microbiome, which can be used, for example, for subsequent testing of antibiotic sensitivity, drug resistance, or for lung microbiome transplantation.