An array substrate, a manufacturing method thereof, and a display device are provided. The array substrate includes: a base substrate; a light-emitting functional layer including a first light-emitting functional portion and a second light-emitting functional portion adjacent to each other; and a photo spacer (PS), the PS is located between the first light-emitting functional portion and the second light-emitting functional portion, the PS includes a plurality of protrusions protruded in a direction away from the base substrate and a first recess located between adjacent protrusions.