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US Patent 11866773 Isolated oligonucleotides containing modified nucleotides

Patent 11866773 was granted and assigned to Envirologix on January, 2024 by the United States Patent and Trademark Office.

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Is a

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Patent Applicant

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Current Assignee

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Patent Jurisdiction

United States Patent and Trademark Office

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Patent Number

Patent Inventor Names

Stephen A. Judice0

Daniel Shaffer0

Date of Patent

January 9, 2024

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Patent Application Number

175266380

Date Filed

November 15, 2021

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Patent Citations

US Patent 7282328 Helicase dependent amplification of nucleic acids

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US Patent 7662594 Helicase-dependent amplification of RNA

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US Patent 8574847 Use of blocker oligonucleotides in selective amplification of target sequences

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US Patent 9096897 Compositions and methods for quantifying a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide

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US Patent 9322053 Compositions and methods for monitoring in real time a nicking amplification reaction comprising a primer oligonucleotide with a 3′—terminal region comprising A2′—modified nucleotide

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US Patent 9631231 Compositions and methods for amplifying a nucleic acid sequence comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide

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US Patent 9845510 Compositions and methods for rapid detection of

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US Patent 10077467 Compositions and methods for detecting a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide

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Patent Primary Examiner

Kenneth R Horlick

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CPC Code

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Patent abstract

Described and featured herein are isolated oligonucleotides containing, from 5′ to 3′, a nicking enzyme recognition sequence, at least 9 nucleotides that specifically bind a target nucleic acid molecule, and 2′ modified nucleotides. The isolated oligonucleotides may be used in compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a Nicking and Extension Amplification Reaction (NEAR) reaction.

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