A handlebar fixing device and a tool cart are disclosed. The handlebar fixing device includes a connecting assembly detachably connected to an enclosure net adjacent to a handlebar, and a fixing assembly arranged on the side of the connecting assembly adjacent to the handlebar, where the fixing assembly is used to fix the handlebar at the fixing assembly. The tool cart includes the handlebar fixing device. When the handlebar rests on the enclosure net, the handlebar is fixed at the fixing assembly and fixed to the enclosure net through the connecting assembly, so that vibration and collision are not easy to cause the handlebar to turn over, thereby reducing the risk of use.