A driving system and a driving method of a display panel are provided. A flash memory is disposed in a first circuit board of the driving system, and a timing controller is disposed in a second circuit board of the driving system. In this way, only one flash memory is needed to store basic configuration data of the timing controller and adjustment data of the display panel, and a number of flash memory is decreased in comparison with two flash memories required in conventional technology. Flash memory capacity is reasonably used, preventing a waste of flash memory capacity while saving production cost.