US Patent 12105270 Low numerical aperture lens based oblique plane illumination imaging

An imaging system includes a first finite conjugate objective at a frontal end of the system and a second finite conjugate objective at a distal end of the system. The system also includes a beam splitting or merging element positioned between the first finite conjugate objective and the second finite conjugate objective. The system also includes an excitation unit configured to direct an excitation beam into a sample positioned in front of the first finite conjugate objective. The excitation beam is in the form of an excitation plane. The system also includes an image sensor positioned facing the second finite conjugate objective. The image sensor lies in a conjugate plane of an excitation beam illumination plane at the frontal end of the system.