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US Patent 12129494 T7 RNA polymerase variants

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Is a

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Patent attributes

Patent Jurisdiction

United States Patent and Trademark Office

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Patent Number

Patent Inventor Names

James Nicholas Riggins0

Yasushi Saotome0

Xiang Yi0

Jared Davis0

Melissa Ann Mayo0

Chinping Chng0

Jonathan S. Penfield0

Oscar Alvizo0

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Date of Patent

October 29, 2024

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Patent Application Number

175584540

Date Filed

December 21, 2021

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Patent Citations

US Patent 7620502 Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedure

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US Patent 9193959 T7 RNA polymerase variants with enhanced thermostability

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US Patent 9260703 Methods of detecting transcription

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US Patent 9447388 DNA polymerases

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US Patent 9499811 Ligation enhancement

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US Patent 9540671 Capping prone RNA polymerase enzymes and their applications

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US Patent 9593326 Protein variant generation by region shuffling

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US Patent 9663769 T7 RNA polymerase variants with cysteine-serine substitutions

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Patent Primary Examiner

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CPC Code

0

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Patent abstract

The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

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