A toothbrush liner for a toothbrush holder includes an elongate pouch sized for insertion into a toothbrush receptacle. A positioner is provided for supporting a toothbrush handle within the pouch and receptacle with a handle end in a spaced apart relationship with a pouch closed end thereby providing a reservoir for accumulation of liquid draining from the toothbrush after use. Alternatively, the liner may include a support web disposed adjacent a closed end of the liner for supporting the toothbrush handle end in a spaced apart relationship with a closed end of the liner in order to provide a reservoir or accumulation of liquid. The liners are disposable and accordingly provide for sanitary use of toothbrushes.