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Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Vellimedu Kannappa Srinivas0
Krishna Ella0
Mosuvan Kuppusamy0
Subhra Lahiri0
Ghan Shyam Khatri0
Date of Patent
September 12, 2006
Patent Application Number
09882509
Date Filed
June 15, 2001
Patent Primary Examiner
Patent abstract
The present invention is based on the isolation of a gene coding for streptokinase from Streptococcus equisimilis (ATCC 9542), its cloning, and expression in E. coli. Two different strategies were carried out for the production of enzymatically-active streptokinase. In the first strategy, streptokinase was expressed as an inclusion body without its signal sequence followed by purification, solubulization, and renaturation to obtain an active preparation. The purified enzyme was formulated and lyophilized. In the second strategy, enzymatically-active streptokinase is secreted into the culture medium. The enzyme was purified, formulated and lyophilized.
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