Patent attributes
The invention is an assay for detecting inhibitors of the enzyme myeloperoxdase The assay method comprises reacting myeloperoxdase with hydrogen peroxide and a chloride source to generate hypochlorous acid in the presence of a potential inhibitor of said myeloperoxidase; reacting any formed hypochlorous acid with an amine to form the corresponding chloramino; optionally removing any unreacted hydrogen peroxide; reacting any for-med chloramine with a detector compound in the presence of iodide to form oxidized detector compound; determining the amount of formed oxidized detector compound by measuring the absorbents or fluorescence at a suitable wavelength; and identifying as inhibitors of myeloperoxidase those compounds which cause the amount of formed oxidized detector compound to be decreased The assay is useful in diagnostic tests for myeloperoxidase activity.
