US Patent 7109008 L-glutamate oxidase
Patent 7109008 was granted and assigned to Yamasa Institute on September, 2006 by the United States Patent and Trademark Office.
All edits
The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties:(A) action: catalyzing the following reaction:L-glutamic acid+O2+H2O→α-ketoglutaric acid+H2O2+NH3;(B) substrate specificity: being specific to L-glutamic acid;(C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000;(D) optimum pH: around pH 6.0 to 8.5;(E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and(F) coenzyme: flavin adenine dinucleotide (FAD).
US Patent 07109008 L-glutamate oxidase US Patent 7109008 L-glutamate oxidase
US Patent 07109008 L-glutamate oxidase
Find more entities like US Patent 7109008 L-glutamate oxidase
