The object of the invention is to provide a handy type visually determinable ATP measuring reagent which can be easily handled and has good measuring sensitivity. The invention is an ATP measuring reagent which rendered possible visual determination by amplifying the reaction to form glucose 6-phosphate from glucose through the joint use of ATP contained in a sample and acetyl phosphate contained in the reagent with glucokinase and acetate kinase contained in the reagent, and further leading it to a visualizable color reaction by the combination of glucose-6-phosphate dehydrogenase and diaphorase.