Is a
Patent attributes
Patent Jurisdiction
Patent Number
Date of Patent
December 5, 2006
Patent Application Number
10670626
Date Filed
September 25, 2003
Patent Primary Examiner
Patent abstract
Methods for making siRNA comprise recombinantly coexpressing a Dicer protein with an R2D2 protein to form a complex comprising the R2D2 protein and the Dicer protein, and contacting the complex with a double-stranded (ds) RNA under conditions wherein the complex cleaves the dsRNA into siRNA. The R2D2 protein may be a Drosophila R2D2 protein and the Dicer protein may be a Drosophila Dicer-2 protein. The Dicer protein and the R2D2 protein may be coexpressed in insect cells, such as S2, Sf9 or Hi5 cells, using a baculovirus expression system.
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