Is a
Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Dian D. Ilsley0
Date of Patent
April 17, 2007
0Patent Application Number
102223680
Date Filed
August 16, 2002
0Patent Citations Received
0
Patent Primary Examiner
Patent abstract
Synthesis of the second strand of cDNA using pol ∝-primase is provided. Pol ∝-primase is used to initiate primers on a cDNA strand de novo, followed by extension of the primer with pol ∝-primase, a second DNA polymerase or a combination thereof. In a preferred embodiment, pol ∝-primase extends the primer, followed by addition of a second DNA polymerase, preferably Klenow fragment.
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