Patent 7211382 was granted and assigned to Orchid Cellmark on May, 2007 by the United States Patent and Trademark Office.
The present invention provides methods and compositions that reduce target-independent primer extension or enhance template dependent primer extension. The methods and compositions of the present invention are applicable not only in PCR, but also in nucleic acid sequencing, genotyping, and other applications employing extension of a primer in a target-dependent manner.