There is provided a system for protection/isolation against infections or disease-bearing persons including an enclosure defining at least two chambers, an isolation chamber having at least one entering and exiting closable opening, at least one ambient air inlet and at least one air outlet connectable to a blower/filter, and an airlock chamber juxtaposed the opening having an access and egress closable aperture, means for forming under-pressure in the isolation chamber and airlock chamber, and at least one biological filter operationally connected to the means for forming under-pressure. A method for protecting/isolating against infection or disease-bearing persons, is also provided.