Patent 7390636 was granted and assigned to NEW ENGLAND BIOLABS, INC. on June, 2008 by the United States Patent and Trademark Office.
Methods and compositions are provided relating to production of recombinant protein in yeast. A modified PLAC4 is described where one or more mutations may be introduced into the Pribnow box-like sequences in the promoter. The modified promoter when placed upstream of a target gene in a vector causes a significant reduction of target gene expression in transformed bacteria but produces efficient expression of the target gene in yeast.