Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Harry van Lune0
Johan Jochem Bruggeman0
Date of Patent
September 16, 2008
Patent Application Number
11433791
Date Filed
May 12, 2006
Patent Primary Examiner
Patent abstract
The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
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