Patent 7449335 was granted and assigned to J.R. Simplot Company on November, 2008 by the United States Patent and Trademark Office.
The present invention provides methods for enhancing plant transformation. One such method entails making an excision in a selected seedling at the point where two cotyledons meet, and then vortexing the cut seedling in a solution comprising a transforming bacterium such as Agrobacterium.