Patent attributes
Contamination by dust particles, bacteria and so forth in each processing step can be reduced by a simple constitution. A culture vessel is used in which a medium vessel in which a medium is sealed and a waste medium vessel capable of housing the fluid in a main culture vessel are connected to the main culture vessel by connecting lines capable of restricting the flow of fluid between them in a state in which they are sealed from the outside. A culture apparatus is composed in which the inner volume of each vessel is variable, and is provided with a case provided with indentations for housing each vessel, and a pressing means that contracts the vessels housed in the indentations by applying external pressure.