Patent 7498135 was granted and assigned to National Institute of Radiological Sciences on March, 2009 by the United States Patent and Trademark Office.
The present invention provides a method for preparing a gene expression profile with a high precision, by carrying out the annealing of primers to templates in the temperature range of TmMAX+6° C. to TmMAX+14° C. of the primer in a PCR step of a HiCEP method.