Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Masanori Hirano0
Date of Patent
March 17, 2009
0Patent Application Number
110788410
Date Filed
March 10, 2005
0Patent Primary Examiner
Patent abstract
An objective of the present invention is to provide methods for synthesizing polynucleotides comprising an unknown nucleotide sequence in a region containing their 5′-end. By using a single gene-specific primer, nucleotide sequences complementary to each other are added respectively at the 5′-and 3′-ends of a desired polynucleotide, to synthesize a desired polynucleotide. Requisite nucleotide sequences can be easily added by incubating with a primer, double-stranded DNA, and template-dependent DNA polymerase. By applying the present invention to 5′ RACE, genes expressed in a low-level, which cannot be obtained by known methods, have been efficiently obtained.
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