A holder for a conventional high intensity focused ultrasound (HIFU) probe to be interfaced with a stepper atop a conventional tripod, or the like, to enable a physician/surgeon to image and visually inspect the rectal and prostate areas of a patient for signs of cancer and other abnormalities so that a treatment plan can be outlined and implemented. The probe holder includes a base, a pair of wall members detachably connected to one another at the front end of the base, and a pair of stand members detachably connected to one another at the rear end of the base. A first opening is formed between the pair of wall members to enclose and reliably support the proximal end of the probe. A second opening is formed between the pair of stand members to enclose and reliably support the distal end of the probe. A continuous cable passageway is established at the rear end of the base and past the lower stand member to permit the power cable to the probe to rotate freely in the event that the probe is correspondingly rotated relative to the base.